Creation of pepper doubled haploids and morphological characterization of androgenic plants

Abstract The Balkan pepper breeding program aims to use in vitro embryogenesis (anther culture) develop doubled haploid lines with valuable traits. We analyzed the effectiveness of microspore on 17 genotypes comprised varieties, lines, and F 1 hybrids different varietal types (conical, bell shape, kapia) characterized agronomic traits newly generated lines. highest androgenic potential was observed variety Stryama line 560/06 9.55 13.00% reacted anthers, respectively. Of 186 regenerated plants, 147 were successfully adapted a survival index 79.03%. Flow cytometry analysis showed that haploid:diploid ratio regenerants 1.5:1. All diploid plants confirmed be origin. There significant differences terms quantitative fruit characteristics length, width, weight, pericarp thickness, productivity per plant among Also, 21, 23, 74 derived from 55 obtained Zlaten medal 7 distinguished higher values some compared initial genotypes. Additionally, several androgenesis (Stryama 74, 55) scored for genotype. These results show anther culture is promising tool creation improved